IIS 401 Error on New Website

I had a new site setup on IIS and thought I got everything setup fine. Everything seems ok from:

• Binding
• SNI
• Certificate
• Enable Allow Anonymous
• Directory permission
• Redirect
• etc

Guess what? Can't even access my home page.

Problem

The server throws the following error:

401 - Unauthorized: Access is denied due to invalid credentials.

You do not have permission to view this directory or page using the credentials that you supplied.

Usually it is because of permission on the directory. But this time it looks fine. Weird thing is I can visit aspx page, but some of the static files are blocked.

Solution

After some browsing, I noticed the value under Site > Authentication > Anonymous Authentication > Edit... (right-click) of the other site that works fine was set to Application pool identity. Meanwhile, my new site was set to Specific user: IUSR and my directory only allow access to IIS_IUSRS.

In my case, setting the above to Application pool identity solves my issue.